Details the leica tcs sp5 has a system magnification of 3. The leica confocal microscope system b46 weill hall revised65 startup turn on 3 red buttons on far right. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. After a short moment you will get the leica application suite start screen. The integration of leica hcs a into the confocal and widefield systems of leica microsystems allows you to standardize biological applications for rapid and reproducible results. Open the leica confocal las af software it takes a few minutes to boot up if you want to use the resonant scanner for live cell imaging check the box, otherwise make sure it is not checked no need to click on microscope or configuration. Updated 8272010 leica tcs sp5 confocal microscope 341 h please contact dr. The leica sp5 is a ultrahigh speed inverted confocal microscope, featuring 5 simultaneous channels, each driven by any of 7 separate laser beams. The confocal system is equipped with multiplel aser lines, including a 405 diode, an argon laser 458, 476, 488, 496,514nm and a white light laser 470670nm in 1 nm increments. In addition to standard pmts this system has hyd detectors gaasp that combine highsensitivity and low noise.
Leica sp5 tile scan protocol 1 open the las af software lower stage completely. Leica confocal tcssp5 this is part of the backprojected pinhole calculator. Pcic sp5 confocal microscope user manual, helene javot updated by mamta srivastava 2112017, page 716 confocal acquisition adjusting the parameters for pmt1, pmt3 and pmt5 go to the acquire tab of the las af software. Leica sp5 live upright reservations igc facilities webpage. Microscope imaging software products leica microsystems. Leica dmi6000cs inverted live cell chamber ludin cube metal halide fluorescence source motorized scanning stage tandem scanners select at software initialization conventional scanner resonant scanner 8 000 hz lasers.
This leica sp2 platform is equipped with two leica confocal microscopes. Leica dmi6000 inverted and automated microscope with a motorized xystage. Data from other instruments can be imported as tiff images. Details the leica tcssp5 has a system magnification of 3. The leica sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. Fill out the logbook and comment on the performance of the system or failure description. The system was designed for confocal scanning laser scanning images of fluorescence. The emission is separated across the entire visible spectrum and each channel has a set of gates that. Leica sp5 laser scanning confocal microscope system. Las x life science microscope software platform products leica. Starting the lcs software on the leica tcs sp5 confocal microscope. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence. Leica sp5 confocal microscope four laser uv, argon, dpss, hene. In the acquire tab, choose a saved or preset laser beam setting in the leica settings window.
This will activate the specified laser lines, load the appropriate dichroic mirror, and set the pmt detectors to. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions. This system has a tandem scanner that has both a standard scanner lower speed, resolution up to 8k x 8k and a resonant scanner higher speed, resolution up to 1k x 1k. To maintain information on the most current level, the description of software functions was. Leica sp5 inverted confocal microscope with aresonant scanner allowing highspeed confocal imaging and is well suited for livecell imaging and highresolution morphological imaging. Confocal microscope system with a broad range of scan speeds suited for. Fan on turn laser power level to lowest setting min turn argon laser key to start briefly, and then back to on turn green and red hene lasers on with keys if needed. Observation modes in addition to confocal imaging are brightfield and epifluorescence. Quick operation manual for leica sp5 confocalmultiphoton. Fluorescence lifetime imaging was performed on the penn vet imaging cores leica sp5 ii inverted confocalflim microscope with a 63x water immersion lens by scanning the sample with a 405 nm pulsed diode laser. Leica tcs sp5 the newest generation of leica confocal microscopes the zmb owns an inverted leica tcs, a tsc sp2 confocal system with upright and inverted microscope stand and an inverted leica tcs sp5. With an intuitive user interface and straightforward navigation, it guides the user through any workflow, whether fast image acquisition or sophisticated expert analysis.
Conducting a sequential scan using the leica tcs sp5 confocal microscope. Instructions for the leica sp5 ii laser scanning confocal microscope. The leica sp5 has four laser lines 405,488458, 476, 496, 514 561, and 633nm and is capable of capturing 5 channels which includes a dic brightfield detector. Confocalcheck analyses the test images and provides a range of output files. The leica tcs sp5 ii features the broadest band of imaging speeds and resolutions ever available in one confocal microscope from 1 to 16,000 lines per second and up to 64 megapixels per image. Introduction to confocal laser scanning microscopy leica. An introduction from your bosssupervisor is not enough the system has 5 detectors. Combine the flexibility of a pointscanning confocal with the high speed of a camerabased widefield system in the same assay to save time. Leica tcs sp5 confocal laser scanning microscope user guide.
The system is equipped with an inverted microscope encased with an incubator for livecell imaging. The leica sp5 confocal microscope is equipped with a spectral head that. Researchers have the option of using three different lasers an argon, krypton, and a heliumneon laser to excite fluorescently. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Leica sp5 x confocal microscope the imaging cores sp5 x confocal microscope arrived in january 2009 and is now available for use. Open source free software for a leica tcs sp5 confocal microscope. Conducting a zstack scan using the leica tcs sp5 confocal microscope. Leica sp5 inverted confocal manual duke light microscopy. With the leica tcs sp5 you can do routine, highspeed and multispectral imaging. Superz galvanometer stage 1500mm travel range3 nm stepsize motorfocus stand. This setup enables you to easily switch between conventional and resonant scanner. Microscope software platform las x for all leica microscopes. How to use leica confocal microscope sp5 external laser part1. The leica sp5 does not use emission filters to define the wavelengths of emission collected, but rather has a prism based approach.
Confocalcheck is not platform specific we have tested the software with leica sp1sp2sp5 systems, a zeiss lsm510 meta and a lsm780 as well as a nikon a1r confocal microscope. Leica sp5 inverted confocal duke light microscopy core facility. Check under configuration that sp5 is selected if not go to configuration and change this to sp5 press ok the confocal software will load now. Hexagonal packing of nuclei red by microtubules green in coeonocytic female gametophyte of ginkgo biloba. The microscope is an upright dm6000 and has a motorized stage. Images were taken on the penn vet imaging cores leica sp5 ii inverted confocalflim microscope with a 100x oil immersion lens by scanning the sample with 488 nm argon and 647 nm hene confocal lasers. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. When asked to initialize the stage, select 2 focus your sample and locate the field of interest.
The free lasaflite software for the sp5 can be downloaded from the facility for imaging by light microscopy london, at the free software for image analysis and visualisation pages. The system provides the full range of scan speeds at the highest resolution. It takes about two minutes for the software hardware to complete the initialization process. The leica tcs sp5 mp covers a wide range of imaging applications multiphoton and one photon by combining two technologies in one system.
If you are the last user fo the day, turn off the complete system incl. You can expand any menu on the left part of the settings screen by clicking on the black arrows. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc. Leica sp5 scanning confocal cdb equipment resource. The leica sp5 confocal is a dmi 6000 model inverted microscope with a super z galvo stage for fast z sectioning and prisms for dic imaging. Leica confocal software lcs lite free download windows. Measuring up to five dimensions, including x, y, z, time and.
Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for. Instead, you are referred to the online help of the leica application suite. What is a confocal microscope webinar leica microsystems. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. Page 1 queens medical research institute college for medicine and veterinary medicine university of edinburgh manual for the leica sp5c spectral confocal laser scanning microscope version 1. National chiao tung university, bio photonics lab, bypraveen kumar. From basic documentation to advanced life science research las x. Las x life science microscope software platform products. Confocal microscope system with a broad range of scan speeds suited for highresolution morphological imaging, as well as multispectral imaging. Microscope imaging software from leica microsystems combines microscope, digital camera and accessories into one fully integrated solution. However, the side length of its square pinhole is usually reported in airy disk units, referenced to a standard wavelength of 580 nm, making it independent of the actual overall magnification.
It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. Leica sp5 inverted confocal duke light microscopy core. Two of these detectors are very sensitive hyd sp gaaspdetectors. Leica confocal software lcs lite free download windows version. Leica confocal tcs sp5 this is part of the backprojected pinhole calculator. Confocal microscope leica tcs sp5 center for advanced.
This video shows how to configure the internal laser power wavelength etc. The following is the details for confocal platform as well as two microscopes. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix checkin and start up. Leica sp5 laser scanning confocal microscope ucsf drc. Lsm 800 with airyscan your compact confocal power pack duration. Leica sp5 ii laser scanning confocal microscope biological. Leica sp5 laser scanning confocal microscope training session. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 6 15 confocal acquisition adjusting the parameters for pmt1, pmt3, pmt4 and pmt5 go to the acquire tab of the las af software. Leica tcs sp5 ii confocal microscopes products home leica leica microsystems.
Leica application suite x las x is the one software platform for all leica microscopes. Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for stitching numerous fields into one large image. Switch speed in an instant the new leica tcs sp5 ii comes with a tandemscanning system mounted on a sliding device. This free program is a product of leica microsystems hd gmbh am friedensplatz 3 d68165 mannheim. How to use leica confocal microscope sp5 external laser. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments. This system has five lasers generating nine lines for fluorescence excitation 405, 458, 476, 488, 496, 514, 543, 594, 633 nm. Leica sp5 laser scanning confocal microscope training. Confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. This 45 minute video is the basic training of the use of the leica sp5 confocal microscope in the mbf. Leica tcs sp5the newest generation of leica confocal microscopes the zmb owns an inverted leica tcs, a tsc sp2 confocal system with upright and inverted microscope stand and an inverted leica tcs sp5. Leica tcs sp5 confocal microscopes products home leica leica microsystems. There are also further links to sp5 compatible software imagej, cellprofiler, leica software, improvision volocity lite you need to register, nice 3d features.
Image zstacks were rendered into the 3d image shown here with volocity software from perkin elmer, accessed through the cores volocity. After the software begins to load youll see text scrolling in the las af start up. Leica tcs sp5 confocal louisiana state university health. Laser point scan confocal with standard and highspeed resonance scanning. Pcmicroscope scanner power laser power laser key las af ok. As a true single point confocal, it perfectly combines fast frame rates, best resolution and depth imaging with full multichannel capabilities. The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. Open source free software for a leica tcs sp5 confocal. Rmbf leica sp5 aobs spectral confocal core facilities.
Conducting a crosstalk test using the leica tcs sp5 confocal microscope. Motorized objective nosepiece 9 mm travel range in z. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. Images courtesy of xiaohong liu, gordon ruthel, and bruce freedman, department of pathobiology, upenn school of veterinary medicine. Leica sp5 laser scanning confocal microscope system listing.